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KMID : 0545120160260061046
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 6 p.1046 ~ p.1056
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RV-23, a Melittin-Related Peptide with Cell-Selective Antibacterial Activity and High Hemocompatibility
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Zhang Shi Kun
Ma Qian
Li Su Bo
Gao Hong Wei
Tan Ying Xia
Gong Feng
Ji Shou Ping
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Abstract
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RV-23 is a melittin-related antibacterial peptide (MRP) with lower cytotoxicity than either melittin or AR-23, another MRP. The aim of this study was to explore the mechanism of RV- 23’s antibacterial selectivity and its hemocompatibility. The results showed that all the peptides exhibited lytic activity against Staphylococcus aureus and Escherichia coli, with RV-23 showing the highest potency. Moreover, RV-23 had lower cytotoxicity than melittin or AR-23 at their minimal inhibitory concentration. In addition, CD experiments showed that melittin, RV-23, and AR-23 all had a typical α-helical structure, and RV-23 had the lowest α-helix content. The structural information showed that RV-23 has the lowest hydrophobicity and highest hydrophobic moment. Because hydrophobicity and α-helix content are believed to correlate with hemolysis, the results indicate that the selective lytic activity against bacteria of RV-23 may be due to its low hydrophobicity and α-helicity, which lead to low cytotoxicity without affecting antibacterial activity. Furthermore, RV-23 did not affect the structure and function of blood components such as red blood cells, platelets, albumin, and the blood coagulation system. In conclusion, RV-23 is a cell-selective antibacterial peptide with high hemocompatibility due to its unique structure.
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KEYWORD
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melittin-related peptide, antibacterial peptide, melittin, AR-23, RV-23, blood compatibility
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